我已经计算了主成分分析(PCA),并得出了PC1与PC2的关系图。当比较三个疾病组(0(对照),1(溃疡性结肠炎)和2(克罗恩氏病))时,这显示了大约14个基因的表达变化。
我想为每组的前三个主要成分制作一个箱形图,总共形成9个箱形图和晶须图。
计算PCA之前的数据矩阵具有与数字0,1或2对应的行名称。这些列代表不同的基因(以及相应的基因表达值)。
我使用prcomp来计算PCA图(按比例缩放,居中并进行对数转换)。
这里是PCA之前我的矩阵的快照;
structure(c(9.11655423831332, 10.489164314825, 1.91402056531454, 7.15827328042159, 4.24137583841638, 8.27769344002199, 8.56104058610663, 10.4808234419919, 2.90978833628418, 6.23818256006594, 5.22964773531333, 10.7708328724305, 7.29461400089235, 11.8318994425553, 3.03424662623575, 8.01272738639518, 4.99017087770597, 11.5985078491858, 7.81888257764922, 11.9022935347989, 1.27378277405718, 7.22371591364402, 5.35032777682152, 11.3245694322554, 7.53493825433311, 12.3702117577478, 2.28591365299837, 6.3684670711928, 4.79325114470697, 11.2368359301193, 7.42400102411584, 10.4893608659259, 2.29357094839174, 7.39880980207098, 4.06127337845416, 10.064874404576, 8.23639009062635, 12.041628287702, 1.68881444318413, 6.83433748681479, 4.58216981866268, 10.7369117797388, 8.52022902181642, 11.8310518930764, 1.09698581801487, 7.01560705946119, 4.42096319700341, 9.55024900954538, 6.78397242802669, 10.7346656491963, 1.8562428132184, 6.79381714159694, 4.76311785326908, 9.2896578696716, 7.38261637784709, 11.8956476271189, 0.676793904156995, 7.12068629785535, 4.50969591112091, 10.3965680730289, 7.76024460081224, 11.4191374294463, 2.51273901194187, 6.49764372886188, 5.95216200154652, 8.80877686581081, 7.92745512232284, 9.64936710370214, 2.75037060332872, 8.32919606967059, 5.13312284319216, 10.0205608136955, 8.32640003009823, 10.7914139100956, 3.07554840032925, 7.71871340592007, 5.75595649315905, 9.71791978048218, 7.13284940508783, 10.9113426747693, 1.07350504928193, 6.56249247218448, 5.35574874951741, 9.54833175767732), .Dim = c(6L, 14L), .Dimnames = list(c("1", "1", "0", "0", "2", "2"), c("Gene1", "Gene2", "Gene3", "Gene4", "Gene5", "Gene6", "Gene7", "Gene8", "Gene9", "Gene10", "Gene11", "Gene12", "Gene13", "Gene14")))
更新;第二个问题已删除。
PCA图的代码如下;
data.mat.1.pca <- prcomp(log(data.mat.1), scale.=T, center=T) pcvalues <- summary(data.mat.1.pca) #colour coding each disease group rownames(data.mat.1) colour_disease <- rownames(data.mat.1) position_control<- grep("0", colour_disease) position_UC<- grep("1", colour_disease) position_Crohn<- grep("2", colour_disease) disease <- vector() disease[position_control] <- "lightskyblue" disease[position_UC] <- "lightslategrey" disease[position_Crohn] <- "lightpink2" ##proportion of variance explained for PC1 and PC2 for plot eigs<- data.mat.1.pca$sdev^2 varExplained.pc1<- round(eigs[1]/sum(eigs), digits=3)*100 varExplained.pc2 <- round(eigs[2]/sum(eigs), digits=3)*100 plot(data.mat.1.pca$x[,1], data.mat.1.pca$x[,2], col=disease, bg=disease, pch=19, cex=1, xlab=paste("PCA 1 (", varExplained.pc1, "%)", sep=""), ylab=paste("PCA 2 (", varExplained.pc2, "%)", sep="")) legend("bottomright", legend = c("Control", "UC", "Crohns"), fill=c("lightskyblue", "lightslategrey", "lightpink2"))
前三台PC的值如下;
PC1 PC2 PC3
S.D 3.6619 0.44801 0.30046
Proportion of Variance 0.9578 0.01424 0.00645
Cumulative proportion 0.9578 0.97215 0.97860
他们正在比较对照与治疗,而我需要三个箱形图(每组一个)。
我已经计算了主成分分析(PCA),并得出了PC1与PC2的关系图。当比较三个疾病组(0(对照),...
绘制像这样的组件分数是很奇怪的,但是尝试下面的方法来获得您提到的组合的点图: