创建 qiime2 清单文件的 python 脚本中的语法错误

Question

我的脚本创建了一个清单文档，其中包含我的 fastq 数据的文件路径，以便上传到 qiime2。输入是样本名称的文本文件和包含 fastq 文件的目录。然后我使用

python ./manifest_single.py --input-dir seqs

运行 shell 文件。输入文本文件示例：

SRR123456 SRR123457 SRR123458...

下面是我的shell文件

#!/local/cluster/bin/python3

# assign a variable to the file of interest
file_name = "seq.by.sys_accession.txt"
dir = "/filepath/seqs"

# open the file with a file handle
read_SRR = open(file_name, "r")

# obtain a list of lines in your file
allLines_list = read_SRR.readlines()

# create output file
outFile = "manifest.txt"

read_outFile = open(outFile, "w")
read_outFile.write(f"sample-id\tabsolute-filepath\n")

# loop over the body of data lines until the end of the file
for rawLine in allLines_list:
    line = rawLine.strip()
    print(f"|{line}|")
    with open(outFile, "a") as f:
        read_outFile.write(f"{line}\t{dir}/{line}.R1.fastq.gz\n")

outFile.close()
read_SRR.close()

但我一直收到错误消息

[name@congo metagenome-analysis]$ python ./manifest_single.py --input-dir seqs
  File "./manifest_single.py", line 17
    read_outFile.write(f"sample-id\tabsolute-filepath\n")
                                                       ^
SyntaxError: invalid syntax

我用文字“制表符”更改了

\t

和

\n

。我已经三次检查了我的输入文件结构和内容。